Real Time qPCR
The QPCR-500 qPCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size.
This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimising the plate size also significantly improves thermal uniformity.
A minimum volume of 5μl is validated, resulting in a more efficient use of expensive and ‘hard to acquire‘ template DNA samples. The QPCR-500 system offers the qPCR capabilities of larger instruments in a compact, accurate footprint. Innovative features include a precise thermal system for unrivalled temperature control, an advanced optical system for highly sensitive fluorescence detection, a 48-well plate for flexible sample throughput and intuitive, icon-driven software for error-free instrument operation.
- MIQE compliant
- HRM functionality is provided as standard and can discriminate class IV SNP 99.9% of the time
- The QPCR-500 can utilise four colours for easy multiplexing
- Industry leading ±0.1ºC temperature uniformity (recorded at 95ºC no settle time)
- High uniformity provides high quality data
- Fast cycling enables several experiments per day, all at an economical price
- Fastest block-based real-time PCR system with the ability to run 40 cycles in 20 minutes (or less when optimised)
- Open platform that can utilise any chemistry, dye or PCR reagent
- Calibrated for SYBR®, FAMTM, HEXTM, VICTM, ROXTM and Cy®5 fluorescent dyes
- Easy to use software, streamlined for novices and experts
- No need to run triplicates, to compensate for poor thermal uniformity of block
Workflow based on
3 simple steps
- Load samples into the 48-well plate using the backlit Eco Dock and place the plate into the Eco 48.
- Run experiment.
- The Eco 48 software provides instrument control, data collection, and data analysis. The most common qPCR parameters are automatically in place. These can be easily changed on the computer to meet specific experimental requirements.