Photometer
DEN-600 is a compact, portable, rechargeable battery powered photometer. The device serves as an inexpensive alternative to a spectrophotometer.
DEN-600 comprises of 600 nm wavelength optical system, which enables to apply – 1) OD600 method that estimated total number of cells, 2) McFarland (McF) turbidity measurement method, 3) Bradford protein assay method for protein concentration measurement.
The device serves as an inexpensive alternative to a spectrophotometer, which is commonly used for these applications. Because DEN-600 is battery powered and compact, it can be comfortably located in a biosafety cabinet, anaerobic chamber or quickly moved to another lab room. Additionally, the vessel holding mechanism allows accommodating standard 10 mm path cuvettes, round bottom, conical vials or falcon tubes, therefore enabling to measure the absorbance and turbidity in Abs, OD or McF units.
USB connectivity and DEN software allow for data transfer, data processing and calculation, software calibration for Bradford protein assay method or a custom calibration for a specifically applicable vessel and custom turbidity standards.
Specifications
| Light source | LED, self-calibrating |
|---|---|
| Photodetector | Silicone photodiode |
| Measurement wavelength (λ) | 600 nm ±10 nm |
| Vessel type | Cuvettes, round bottom tubes, falcon tubes |
| Measurement modes | Absorbance (Abs), McFarland (McF) |
| Measurement range | 0 – 3.0 Abs | 0 – 16.00 McF |
| Resolution | 0.001 Abs | 0.01 McF |
| Accuracy | ±0.006 @ 1 Abs | ±0.1 @ 0-8 McF |
| Repeatability | ±0.003 @ 1 Abs | ±0.05 @ 0-8 McF |
| Battery type | Li-Ion |
| PC system requirements: | Intel/AMD Processor, 1 GB RAM, Windows Vista/7/8/10, USB |
| Dimensions (W×D×H) | 120 × 145 × 65 mm |
Common applications include
Cell concentration measurement
Cell growth data estimation
Log phase estimation for microbial cells induction
Competent cell preparation
Bradford protein assay method
Antibiotic susceptibility testing
Inhibitory tests